Enzyme Lab Report

3 Pages 649 Words

Enzyme Activity
Abstract:

The purpose of this experiment is to test the effects of different sugar types with pH and temperatures. We are testing to see if there is a difference in reaction and reaction time when placed into different temperature and pH. We are looking for changes in color, solidity, reaction time, etc. My hypothesis is that at different temperatures and amounts of pH, there will we different reactions.

Introduction:

The overview of these experiments is to test enzymes and see what kinds of reactions they have in different setting. Temperature, pH, sugars, etc. are all part of this testing process. The importance of this experiment is to gain better knowledge of enzymes so that we can know more about them. Enzymes are very important in everyday life because they keep people from getting diseases and they help us to do simple thing like digest foods.

Procedure:

Collect lab supplies and begin testing. First we mix 2ml of each sugar with 2ml of Benedicts Solution. We then placed these tubes in hot water and after three minutes noted a color change. The point of this test was to find out which were single sugars and which were double sugars. After everything we record our findings.
For our next test we take the same amounts of sugar and Benedicts solution but we increase the enzyme to see if the amount of the reaction will increase. We mix the correct solution of sulfur , sucrose solution, enzyme stock solution and water and let sit at room temperature for fifteen minutes. We then record our findings.
Our next test will be the effects of temperature on enzyme activity. We place buffer, enzyme, and sucrose solutions into four different temperature settings. We then mix them after six minutes. We let them sit for another fifteen minutes and record our findings.
Next we test the effects of pH on enzymes. We first add 5ml of enzyme stock solution to form different pH levels all 2ml ...